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cellengine cloud based flow cytometry analysis software  (CellCarta)
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Changes in intratumoral and systemic inflammation after vancomycin preconditioning followed by SER-401 and nivolumab (vs. placebo control). A, There was a detectable increase in tumor-infiltrating CD8 cells at C2D1 compared with baseline, with an increased percentage in a pooled analysis ( P = 0.06, Mann–Whitney test). B, In patients with available matched baseline and on-treatment samples ( n = 3), there was an increase of intratumoral CD8 T-cell infiltration on treatment. Representative IHC images are shown, 1 per arm. C, Comparison of circulating immune cell populations representing change from baseline to D-7 in the SER-401 arm versus the placebo arm by dimensional flow <t>cytometry.</t> Left, Red horizontal line on the volcano plot indicates statistical significance ( P < 0.01). Right, Three immune cell populations (1: PD-1 + TIGIT + Treg; 2: PD-1 + Treg; 3: FOXP3 − PD-1 + effector cells) from volcano plot over time as mean fold change over baseline. P values represent statistical significance as compared with the baseline by the Mann–Whitney test. D, Comparison of circulating proteins between SER-401 and placebo arms from baseline to D-7 (left) and baseline to C1D1 (right). The red horizontal line on the volcano plot indicates statistical significance ( P < 0.05).
Cellengine Cloud Based Flow Cytometry Analysis Software, supplied by CellCarta, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Changes in intratumoral and systemic inflammation after vancomycin preconditioning followed by SER-401 and nivolumab (vs. placebo control). A, There was a detectable increase in tumor-infiltrating CD8 cells at C2D1 compared with baseline, with an increased percentage in a pooled analysis ( P = 0.06, Mann–Whitney test). B, In patients with available matched baseline and on-treatment samples ( n = 3), there was an increase of intratumoral CD8 T-cell infiltration on treatment. Representative IHC images are shown, 1 per arm. C, Comparison of circulating immune cell populations representing change from baseline to D-7 in the SER-401 arm versus the placebo arm by dimensional flow cytometry. Left, Red horizontal line on the volcano plot indicates statistical significance ( P < 0.01). Right, Three immune cell populations (1: PD-1 + TIGIT + Treg; 2: PD-1 + Treg; 3: FOXP3 − PD-1 + effector cells) from volcano plot over time as mean fold change over baseline. P values represent statistical significance as compared with the baseline by the Mann–Whitney test. D, Comparison of circulating proteins between SER-401 and placebo arms from baseline to D-7 (left) and baseline to C1D1 (right). The red horizontal line on the volcano plot indicates statistical significance ( P < 0.05).

Journal: Cancer Discovery

Article Title: Randomized Placebo-Controlled, Biomarker-Stratified Phase Ib Microbiome Modulation in Melanoma: Impact of Antibiotic Preconditioning on Microbiome and Immunity

doi: 10.1158/2159-8290.CD-24-0066

Figure Lengend Snippet: Changes in intratumoral and systemic inflammation after vancomycin preconditioning followed by SER-401 and nivolumab (vs. placebo control). A, There was a detectable increase in tumor-infiltrating CD8 cells at C2D1 compared with baseline, with an increased percentage in a pooled analysis ( P = 0.06, Mann–Whitney test). B, In patients with available matched baseline and on-treatment samples ( n = 3), there was an increase of intratumoral CD8 T-cell infiltration on treatment. Representative IHC images are shown, 1 per arm. C, Comparison of circulating immune cell populations representing change from baseline to D-7 in the SER-401 arm versus the placebo arm by dimensional flow cytometry. Left, Red horizontal line on the volcano plot indicates statistical significance ( P < 0.01). Right, Three immune cell populations (1: PD-1 + TIGIT + Treg; 2: PD-1 + Treg; 3: FOXP3 − PD-1 + effector cells) from volcano plot over time as mean fold change over baseline. P values represent statistical significance as compared with the baseline by the Mann–Whitney test. D, Comparison of circulating proteins between SER-401 and placebo arms from baseline to D-7 (left) and baseline to C1D1 (right). The red horizontal line on the volcano plot indicates statistical significance ( P < 0.05).

Article Snippet: Data were analyzed using CellEngine cloud-based flow cytometry analysis software (CellCarta).

Techniques: Control, MANN-WHITNEY, Comparison, Flow Cytometry